Journal: Journal of visualized experiments : JoVE

Article Title: Isolation of Brain Microglia to Study Persistence of Human Immunodeficiency Virus-1

doi: 10.3791/71673

Figure Lengend Snippet: ( A-C ) Flow cytometry analysis of CD3 + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, CD3 + T cells, and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .

Article Snippet: CD3 MicroBead Kit, non-human primate , Miltenyi Biotec , Cat# 130-092-012 , .

Techniques: Isolation, Flow Cytometry, Analysis

Journal: Frontiers in Veterinary Science

Article Title: Bleomycin-based electrochemotherapy for recurrent oral CD8-positive epitheliotropic T-cell lymphoma in a dog with durable local control and necrotic regional lymph node changes: a case report

doi: 10.3389/fvets.2026.1942467

Figure Lengend Snippet: Immunohistochemical findings of the upper lip mucosal lesion. (A) CD3 immunostaining. (B) CD8α immunostaining using the rat monoclonal antibody clone F3-B2. More than 90% of the neoplastic lymphocytes were positive for both CD3 and CD8α.

Article Snippet: CD3 immunohistochemistry was performed using a rat anti-human CD3 monoclonal antibody (clone CD3-12; Bio-Rad), which is cross-reactive with canine CD3.

Techniques: Immunohistochemical staining, Immunostaining

Journal: Frontiers in Veterinary Science

Article Title: Bleomycin-based electrochemotherapy for recurrent oral CD8-positive epitheliotropic T-cell lymphoma in a dog with durable local control and necrotic regional lymph node changes: a case report

doi: 10.3389/fvets.2026.1942467

Figure Lengend Snippet: Histopathological findings of the excised left mandibular lymph node. Hematoxylin and eosin (HE) stain. (A) Low-power view showing extensive necrosis replacing most of the normal lymph node architecture. Scale bar = 1 mm. (B) High-power view of the necrotic area. Scale bar = 50 μm. (C) Small residual aggregate of lymphoid cells identified within the excised lymph node. Scale bar = 50 μm. (D) CD3 immunostaining. Although the residual lymphoid cells were CD3-positive, extensive necrosis precluded definitive determination of lymphomatous involvement. Scale bar = 50 μm.

Article Snippet: CD3 immunohistochemistry was performed using a rat anti-human CD3 monoclonal antibody (clone CD3-12; Bio-Rad), which is cross-reactive with canine CD3.

Techniques: H&E Stain, Immunostaining

Journal: Frontiers in Immunology

Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

doi: 10.3389/fimmu.2026.1833383

Figure Lengend Snippet: Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of CD8 + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.

Article Snippet: Antibodies included: FITC Mouse monoclonal to CD68 (ab134351, abcam, UK), Pacific Blue anti-human CD86 Antibody (305417, yajimall, China), CD206 (MMR) Monoclonal Antibody (19.2)-PE (12-2069-42, ThermoFisher, USA), PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody (E-AB-F0995J, Elabscience, China), PE Anti-CD206 mouse antibody (E-AB-F1135D, Elabscience, China), APC Anti-mouse CD3 antibody (E-AB-F1013E, Elabscience, China), PE Anti-mouse CD8 alpha antibody (E-AB-F1104D, Elabscience, China).

Techniques: Over Expression, Cell Culture, Flow Cytometry, Co-culture Assay

Journal: Frontiers in Immunology

Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

doi: 10.3389/fimmu.2026.1833383

Figure Lengend Snippet: LINC00887/UPF1-mediated aerobic glycolysis leads to lactic acid accumulation and facilitates immune evasion in ccRCC in vivo , A syngeneic tumor model was developed by injecting BALB/c mice with Renca cells expressing sh-NC or sh-LINC00887, followed by treatment with sh-NC or sh-UPF1 lentivirus. A-C: Assessment of tumor size (A) , volume (B) , and weight (C) . (D, E) qRT-PCR analyses for LINC00887 (D) and UPF1 (E) mRNA levels. (F) IHC analysis for UPF1, LDH-A, KI67 protein levels. (G) Kit assay for lactate levels in tumors. (H) Proportion of M2 macrophages (F4/80+CD206+) by flow cytometry. (I) Immunofluorescence assay for CD206+ macrophages. (J) Proportion of CD8 + T cells (CD3 + CD8 + ) in tumor tissues. ns, no significant difference. * p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; LDH-A, Lactate dehydrogenase A; CD206, Mannose receptor C-type 1; CD3, Cluster of differentiation 3; CD8, Cluster of differentiation 8.

Article Snippet: Antibodies included: FITC Mouse monoclonal to CD68 (ab134351, abcam, UK), Pacific Blue anti-human CD86 Antibody (305417, yajimall, China), CD206 (MMR) Monoclonal Antibody (19.2)-PE (12-2069-42, ThermoFisher, USA), PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody (E-AB-F0995J, Elabscience, China), PE Anti-CD206 mouse antibody (E-AB-F1135D, Elabscience, China), APC Anti-mouse CD3 antibody (E-AB-F1013E, Elabscience, China), PE Anti-mouse CD8 alpha antibody (E-AB-F1104D, Elabscience, China).

Techniques: In Vivo, Expressing, Quantitative RT-PCR, Analysis, Flow Cytometry, Immunofluorescence